《植物生理学报》 2010, 46(8): 803-807

高山离子芥CbMAPK3 基因功能分析

张腾国¹, 夏小慧², 刘玉冰³, 杨宁¹, 贾凌云¹, 王娟¹, 安黎哲^4,*

1 西北师范大学生命科学学院, 兰州 730070; 2 兰州城市学院, 兰州 730070; 3 中国科学院寒区旱区环境与工程研究所, 兰州730000; 4 兰州大学生命科学学院, 兰州 730000

通信作者：安黎哲；E-mail: lizhean@lzu.edu.cn；Tel: 0931-7971722

摘　要：

将高山离子芥丝裂原活化蛋白(MAP)激酶基因CbMAPK3编码区连接到表达载体pET-30a中, 转化大肠杆菌JJ001 (srl:: Tn10), 在 1 mol•L-1 山梨醇的渗透胁迫及 IPTG 的诱导下, 转化 pET-30a-CbMAPK3 的大肠杆菌 JJ001 (srl::Tn10)生长情况比 转化 pET-30a 的好。免疫分析表明, 在 0 ℃下处理时, 高山离子芥 CbMAPK3 激酶蛋白质水平没有明显的变化; 在 4 ℃处理 下, 高山离子芥 CbMAPK3 激酶蛋白质水平在处理前 3 h 明显增加, 并在 3 h 后达到最高值。高山离子芥 CbMAPK3 在响应 环境胁迫的过程中起作用。

关键词：高山离子芥; CbMAPK3基因; 功能分析

收稿：2010-04-06   修定：2010-06-29

资助：国家自然科学基金(30960065 和30800122)和西北师范大学知识与科技创新项目(nwnu-kjcxgc-03-76 和nwnukjcxgc-03-49)。

Functional Analysis of CbMAPK3 Gene in Chorispora bungeana Fisch. et Mey.

ZHANG Teng-Guo¹, XIA Xiao-Hui², LIU Yu-Bing³, YANG Ning¹, JIA Ling-Yun¹, WANG Juan¹, AN Li-Zhe^4,*

¹School of Life Sciences, Northwest Normal University, Lanzhou 730070, China; ²Lanzhou City University, Lanzhou 730070,China; ³Cold and Arid Regions Environmental and Engineering Institute, Chinese Academy of Sciences, Lanzhou 730000, China;⁴College of Life Sciences, Lanzhou University, Lanzhou 730000, China

Corresponding author: AN Li-Zhe; E-mail: lizhean@lzu.edu.cn; Tel: 0931-7971722

Abstract:

Chorispora bungeana is a rare alpine subnival plant species that is highly tolerable to freezing environment. To investigate whether mitogen-activated protein (MAP) kinase CbMAPK3 of C. bungeana has the osmo-protection function, the coding region was subcloned into prokaryotic expression vector pET-30a. In the presence of 1 mol·L^-1 sorbitol and induction with IPTG, Escherichia coli strain JJ001 (srl::Tn10) transformed with pET-30a-CbMAPK3 exhibited better growth than the same strain transformed with the empty vector. Western blot analysis showed that the protein level of CbMAPK3 was not significantly altered by 0 ℃ freezing treatment, but increased significantly at 4 ℃ cold treatment and the maximum level was observed at 3 h after the treatment. These results indicated that the CbMAPK3 might play an role in response to environmental stresses.

Key words: Chorispora bungeana; CbMAPK3 gene; functional analysis